TABLE 2.
Genes upregulated by EHV-1 KyA in the lungs of CBA mice
| Gene function and name | Fold changea in expression at: |
Description | ||
|---|---|---|---|---|
| 6 hpi | 8 hpi | 12 hpi | ||
| Immune and inflammatory responses | ||||
| SLFN4 | 5.5 | 10.7 | 42.4 | Schlafen 4: macrophage activation |
| SLFN3 | 4.1 | 6.2 | 24.9 | Schlafen 3: T cell activation |
| TNFRSF9 | 7.2 | 4.9 | 8.8 | TNF receptor superfamily, member 9: T cell development |
| PTX3 | 9.4 | 8.4 | 9.8 | Pentraxin 3: innate immune response |
| IRG1 | 133.1 | 7.8 | 17.7 | Immunoresponsive gene 1: inhibition of the inflammatory response |
| CD177 | 5.7 | 6.0 | — | CD177 antigen: neutrophil activation |
| S100A9 | 2.2 | 7.4 | 13.1 | S100 calcium binding protein A9: neutrophil chemotaxis and adhesion |
| S100A8 | 2.9 | 7.1 | 12.7 | S100 calcium binding protein A8: neutrophil chemotaxis and adhesion |
| CLEC4D | 5.1 | 5.3 | 7.8 | C-type lectin domain family 4, member d: inflammation and immune response |
| CLEC4E | 10.0 | 4.1 | 7.8 | C-type lectin domain family 4, member e: cell surface receptor for ligands |
| SOCS3 | 3.6 | 4.2 | 5.9 | Suppressor of cytokine signaling 3 |
| LCN2 | 5.8 | 3.6 | 6.5 | Lipocalin 2: macrophage deactivation |
| Orm2 | 31.8 | 5.8 | 7.9 | Orosomucoid 1: immunosuppression |
| CC chemokines | ||||
| CCL2 (MCP-1) | 16.0 | 11.2 | 20.0 | Chemokine (C-C motif) ligand 2 |
| CCL7 (MCP-3) | 14.3 | 8.8 | 19.8 | Chemokine (C-C motif) ligand 7 |
| CCL20 (MIP-3α) | 53.4 | 8.3 | 3.7 | Chemokine (C-C motif) ligand 20 |
| CCL12 (MCP-5) | 7.0 | 4.0 | 8.6 | Chemokine (C-C motif) ligand 12 |
| CXC chemokines | ||||
| CXCL5 | 141.3 | 31.2 | 25.0 | Chemokine (C-X-C motif) ligand 5 |
| CXCL1 | 12.6 | 5.5 | 15.6 | Chemokine (C-X-C motif) ligand 1 |
| CXCL10 | 6.2 | 20.3 | 95.0 | Chemokine (C-X-C motif) ligand 10 |
| CXCL3 (MIP-2β) | 106.9 | 10.2 | 25.5 | Chemokine (C-X-C motif) ligand 3 |
| CXCL2 (MIP-2α) | 50.8 | 11.0 | 12.4 | Chemokine (C-X-C motif) ligand 2 |
| Cytokines | ||||
| IL-6 | 45.0 | 33.2 | 46.4 | Interleukin 6 |
| IL-1β | 11.2 | 8.3 | — | Interleukin 1β |
| IL-1RN | 3.7 | 2.7 | — | Interleukin 1 receptor antagonist |
| IL-10 | 5.5 | — | — | Interleukin 10 |
| IL-11 | 7.2 | — | — | Interleukin 11 |
| TNF | 14.5 | — | 2.2 | Tumor necrosis factor |
| TLR-associated factorsb | ||||
| TLR2 | 3.4 | 2.1 | 3.1 | Toll-like receptor 2 |
| CD14 | 13.2 | 5.9 | 6.0 | CD14 antigen |
| MyD88 | 3.1 | 2.2 | 2.6 | Myeloid differentiation primary response gene 88 |
| Cell cycle and apoptosis | ||||
| Gadd45g | 5.0 | — | 2.9 | Growth arrest and DNA damage-inducible 45 gamma |
| Casp4 | 4.8 | 2.8 | 3.2 | Caspase 4: inhibition of cell growth and induction of apoptosis |
| Antibacterial factors | ||||
| Reg3g | 74.8 | — | — | Regenerating islet-derived 3 gamma |
| Adm | 10.6 | 4.3 | 4.8 | Adrenomedulin |
a
Mean fold change from three replicate experiments at 8 or 12 hpi and from two replicate experiments at 6 hpi. —, fold difference of <±2.0 between infected and mock-infected lungs.
b
TRL, Toll-like receptor.