. 2016 Aug 26;90(18):8090–8104. doi: 10.1128/JVI.00986-16

TABLE 3.

EHV-1 KyA immunization increases the expression of antiviral genes in the lungs of CBA mice challenged with RacL11

Gene name	KyA-infected mice		RacL11-infected mice		KyA → RacL11-infected mice^a		HI-KyA → RacL11-infected mice^b
Gene name	Fold change^c	P value^d	Fold change^c	P value^d	Fold change^c	P value^d	Fold change^c	P value^d
RSAD2	6.2	0.024032	4.7	0.004344	21.6	0.000066	5.0	0.029294
MX1	4.5	0.014696	4.7	0.015646	29.4	0.000057	5.1	0.022906
MX2	2.0	0.046565	2.4	0.010745	5.5	0.000022	-
IRF7	2.3	0.040911	2.7	0.002847	27.8	0.000002	2.2	0.049218
IFI44L	2.3	0.039495	2.4	0.005339	48.2	0.000001	2.8	0.043189
IFIT1	4.9	0.022448	5.2	0.002613	12.9	0.000130	4.7	0.011233
IFIT2	3.4	0.039152	2.7	0.009578	7.9	0.000065	2.4	0.019820
IFIT3	2.7	0.034187	3.1	0.000898	8.5	0.000001	2.8	0.011494
ISG15	3.8	0.027831	4.4	0.004029	19.7	0.000102	2.6	0.028449
ISG20	—		—		3.9	0.000062	—
OAS1a	—		2.0	0.001532	11.1	0.000009	—
OAS2	—		—	0.006082	9.8	0.000087	—
OASL2	2.1	0.025561	2.5	0.000725	8.4	0.000008	—
GBP2	2.0	0.036321	—		4.4	0.000159	—
SLC15A3	—		—		3.1	0.000657	—
IFI204 (IFI16)	3.0	0.053942	2.0	0.062900	29.7	0.000098	2.5	0.038217
IFN-γ	—		—		8.5	0.000413	—

CBA mice immunized with EHV-1 KyA were challenged with pathogenic RacL11 at 3 days postimmunization.

CBA mice immunized with heat-inactivated EHV-1 KyA were challenged with pathogenic RacL11 at 3 days postimmunization.

Mean fold change in expression from three replicate experiments at 8 h postinfection (for KyA- or RacL11-infected mice) or 8 h post-RacL11 challenge (for KyA → RacL11- or HI-KyA → RacL11-infected mice). —, fold difference of <±2.0 between infected and mock-infected lungs.

Statistics for the three replicates of each gene were calculated independently. Mean signals were compared by using a t test analysis assuming equal variances and 6 degrees of freedom.