FIG 2.

Growth kinetics of G2A-GPC and ΔG2-GPC rCan in Vero cells. Separate 6-cm culture dishes were infected at a multiplicity of infection (MOI) of 0.01, and culture supernatants were harvested at the indicated times. Accumulations of infectious particles (A) and NP (B) were determined, respectively, by using a plaque assay and NP ELISA as described in Materials and Methods. The results presented are representative of two independent growth experiments. Black squares, wild-type rCan; green circles, G2A-GPC rCan; red triangles, ΔG2-GPC rCan. (C) Infected cell monolayers were immunochemically stained at the 36-h time point with MAb AG12 to illustrate the comparable extent of virus spread through the cultures, in agreement with results from the NP ELISA. At later time points, infection is too widespread to visually judge the proportion of infected cells. Scale bar, 200 μm. OD, optical density; FFU, focus-forming units.