TABLE 2.
Nucleotide changes in p30 ΔG2-GPC rCan
| RNA nucleotide position | Nucleotide change (% of reads)a | Amino acid changea |
|---|---|---|
| S RNA | ||
| 1–8 | Missing | 5′ UTR |
| 9 | A→ C (93) | 5′ UTR |
| 92–94 | Missing | SSP ΔG2 mutation |
| 198 | A → G (77) | SSP N37S |
| 273 | T → A (21) | GP1 F62Y |
| 285 | T → A (36) | GP1 L66Q |
| 462 | T → C (29) | GP1 I125T |
| 488 | T → C (25) | GP1 W134R |
| 590 | G → A (35) | GP1 A168T |
| 2067 | G → A (100) | NP silent |
| 2070 | A → G (100) | NP silent |
| 2178 | G → A (24) | NP silent |
| 2849 | T → C (42, 76)b | NP K162E |
| 3406 | A → T (56) | 3′ UTR |
| L RNA | ||
| 22 | T → A (100) | 5′ UTR |
| 367–368 | tAG → tGA (100) | Z stop (silent) |
| 452 | T → C (100) | L stop (silent) |
| 1634 | A → G (100) | L silent |
| 2891 | C → A (100) | L L1398F |
| 4305 | C → T (100) | L G927E |
| 4682 | T → G (100) | L silent |
| 4688 | G → A (100) | L silent |
| 4803 | C → T (100) | L R761K |
| 4856 | T → C (49) | L silent |
| 4940 | G → A (100) | L silent |
| 5032 | C → T (55) | L D685N |
| 5296 | G → A (100) | L R597W |
| 5321 | G → A (100) | L silent |
| 6045 | T → A (58) | L N347I |
| 6295 | A → T (57) | L S264T |
| 6814 | G → A (100) | L L91F |
| 7107 | C → T (69) | 3′ UTR |
a
Included are changes present in ≥20% of reads relative to Candid #1 S and L RNA reference genomes (GenBank accession numbers AY746353.1 and AY746354.1, respectively). Changes in the reverse genetics plasmids relative to the reference sequence (J.C. de la Torre, personal communication) are italicized.
b
Percentages of reads in original and p30 populations, respectively.