TABLE 3.
Intracellular dNTP levels in TN and TCM CD4+ T cells in the absence and presence of 100 nM CCL19
| Cell type and treatment | Nucleotide concn (fmol/106 cells)a |
|||
|---|---|---|---|---|
| dATP | dGTP | dCTP | dTTP | |
| TN cells | 8.70 (<4–10.7) | 32.0 (14.6–44.5) | 27.7 (<4–43.1) | 5.90 (<4–7.60) |
| TN cells + CCL19 | 4.70 (<4–6.10) | 28.2 (17.5–29.4) | 14.7 (<4–30.6) | 4.70 (<4–7.30) |
| TN + anti-CD3/CD28 | 379 (112–513) | 484 (404–622) | 88.6 (62.7–111) | 649 (244–735) |
| TCM cells | 3.70 (<4–5.8) | 30.0 (19.5–77.1) | 11.5 (5.75–21.0) | 4.40 (<4–6.90) |
| TCM cells + CCL19 | 4.10 (<4–4.90) | 24.6 (11.6–61.6) | 10.0 (4.80–17.3) | 4.90 (<4–12.3) |
| TCM + anti-CD3/CD28 | 285 (57.2–294) | 232 (217–345) | 41.0 (31.9–61.8) | 164 (106–188) |
a
Data are presented as the median (range) of three independent experiments. A value of <4 indicates a level below the limit of detection based on the number of cells used for quantification.