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. 2016 Aug 26;90(18):8132–8149. doi: 10.1128/JVI.00483-16

FIG 5.

FIG 5

Fusion assay of ASCT2- and Dasy-Env1.1-transfected cocultured cells demonstrates that ASCT2 is the Dasy-Env1.1 receptor. Cell-cell fusion was assayed upon independent transfections of a set of A23 cells with an empty vector (none) or an expression vector for either the Dasy-Env1.1, syncytin-1 (syn1), or syncytin-2 (syn2) protein together with an nls-lacZ gene expression vector and another set of A23 cells with an expression vector for human ASCT2 (hASCT2), Dasypus ASCT2 (dasyASCT2), the human syncytin-2 receptor MFSD2 (38), or an empty vector (none). One day after transfection, cells were resuspended, and pairs of transfected cells from each set were cocultured for 1 to 2 days, fixed, and stained with X-Gal. Syncytia can be easily detected (arrows) for the Dasy-Env1.1/dasyASCT2, Dasy-Env1.1/hASCT2, syncytin-1/hASCT2, syncytin-1/dasyASCT2, and syncytin-2/MFSD2 pairs, with only mononucleated cells being visible in the other cases.