TABLE 1.
Virus | Avg virus titer (mean log10 TCID50/ml ± SD)a |
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---|---|---|---|---|---|---|
1 dpi |
3 dpi |
5 dpi |
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Nasal turbinate | Lung | Nasal turbinate | Lung | Nasal turbinate | Lung | |
Wild type | 2.08 ± 0.29 | 3.08 ± 0.58 | 4.12 ± 0.38 | 5.83 ± 0.38 | 3.42 ± 0.14 | 5.58 ± 0.14 |
PA-K356R | 2.33 ± 0.14 | 3.33 ± 0.14 | 4.67 ± 0.14 | 6.25 ± 0.43 | 4.25 ± 0.66* | 6.42 ± 0.58* |
PB2-E627K | 2.42 ± 0.14 | 3.50 ± 0.25 | 4.83 ± 0.38 | 6.75 ± 0* | 4.75 ± 0.66* | 6.42 ± 0.58* |
PA-K356R/PB2-E627K | 2.67 ± 0.14 | 3.83 ± 0.38 | 5.83 ± 0.14*# | 6.83 ± 0.38* | 5.08 ± 0.29* | 6.50 ± 0.25* |
Lower limits of detection were 101.25 TCID50/ml for nasal turbinates or lungs. * indicates that the virus titer of the corresponding mutant strain was significantly higher than that of the wild-type virus, and # indicates that the virus titer of the corresponding mutant strain was significantly higher than that of the PB2-E627K virus, with a P value of <0.05, as determined by one-way ANOVA.