FIG 2.
Inhibition of CA-SP1 processing by the PF-46396 analogue series as determined by a quantitative CA-SP1 processing assay. HeLa cells were transfected with pNL4-3/WT and cultured with 5 μM compound or an equivalent volume of DMSO. Cells were metabolically labeled for 2 h with [35S]Met-Cys, and released virions were pelleted by ultracentrifugation. (A) Virus lysates were immunoprecipitated with HIV-Ig, and processing of CA-SP1 to CA was analyzed by SDS-PAGE and fluorography. (B) Phosphorimager analysis was used to quantify the percentage of CA-SP1 relative to total CA-SP1 plus CA in pelleted virus samples. Mean values from independent experiments are presented (n = 3), and error bars indicate standard deviations.