Table 3.

Antibodies Used for Antigen Detection

Antigen	Source	Clone	ng/test	Fluorochrome	Excitation	Long‐pass Dichroic Mirror	Bandpass filter
CD45RO	Biolegend	UCHL1	2500	Brilliant violet 421 or Pacific Blue	405 nm	Blank	450/50
CD4	Biolegend	OKT4	150	Brilliant violet 510	405 nm	505LP	525/50
CD56	Biolegend	HCD56	500	Brilliant violet 510	405 nm	505LP	525/50
CD16	Biolegend	3G8	500	Brilliant violet 605	405 nm	595LP	605/40
HLA‐DR	Biolegend	L243	180	Brilliant violet 711	405 nm	685LP	710/50
CD16	Biolegend	3G8	600	Brilliant violet 785	405 nm	750LP	780/60
CD45RA	Biolegend	HI100	400	AF488	488 nm	505LP	530/30
CD3	eBioscience	UCHT1	500	PerCP‐Cy5.5	488 nm	685LP	695/40
CD8	Biolegend	HIT8a	2500	AF700	640 nm	685LP	710/50
Viability marker	eBioscience	Cat# 65‐0865	1:1000	eF780	640 nm	755LP	780/60
FoxP3	BD	259D/C7	500	PE	561 nm	Blank	582/15

Intranuclear antigens are noted in italics.