Bar graphs represent the average and standard deviation for (A) expression of CD107a (B) and levels of IFN-γ, (C) TNF-α and (D) IL17 secretion following a 12 hour stimulation with phorbol 12-myristate-13-acetate (PMA)/ionomycin for the subsets of NK cells in rectal mucosa of naïve, SIV acute and SIV chronically infected rhesus macaques. Peripheral blood mononuclear cells were stimulated with 721.122 cells at E:T ratio of 5:1 for 12 hours and measured (E) CD107a, (F) IFN-γ and (G) TNF-α production on Total, CD16, CD56 and DN NK cells subsets in naïve, acute and chronically SIV infected animals. Data represents the seven animals per group. Kruskal-Wallis tests were used for comparisons of each group. Only significant P values are shown (*P < .05; **P < .01; ***P < .001).