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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: J Immunol. 2016 Apr 13;196(10):4185–4195. doi: 10.4049/jimmunol.1600014

FIGURE 6. IgM seroconversions to Vsp1 and Vlp15/16 in acute HTBRF patients.

FIGURE 6

(A) Western blots on recombinant proteins GlpQ, Vsp1, Vlp15/16 and Vlp18. Strips were incubated in sera from nine Russian patients (Bm1 to Bm9) collected at the day they were first found B. miyamotoi PCR positive in blood, and approximately one week thereafter. IgM was detected and all blots were developed simultaneously. The following blots were considered positive: GlpQ seroconversion: Bm1, Bm2, Bm4, Bm7, Bm8; Vsp1 seroconversion: Bm4 and Bm9; Vlp15/16 seroconversion: Bm1, Bm2, Bm7, Bm8. Vlp18 seroconversion: Bm1.

(B) Sera from Russian patients admitted for suspected tick-borne disease and diagnosed by a physician with erythema migrans (Bb1 to Bb6) were tested for IgM against GlpQ, Vsp1, Vlp15/16 and Vlp18. The following blots were considered positive: GlpQ seroconversion in patient Bb3, GlpQ in acute and convalescent serum of Bb2. Vlp18 in acute and convalescent serum of patient Bb3.