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. 2016 Jun 9;37(9):839–851. doi: 10.1093/carcin/bgw068

Figure 5.

Figure 5.

Microglial NHE1 activation stimulates glioma proliferation. (A) Protocol. GCM were harvested from glioma cultured in a FBS-free medium for 24h (GC#22 or GC#99). Microglia cultures were then exposed to GCM for 24h either in the presence or absence of NHE1 inhibitor HOE642 (1 μM). G/MCM were subsequently harvested and added to glioma cultures for 24h. Regular microglia culture media (C/MCM) and astrocyte-conditioned media (A/MCM) were used as controls. (B) Representative immunoblots showing increased expression of p-Akt in glioma cells in response to G/MCM. Data are mean ± SEM. n = 4, *P < 0.05 versus C/MCM+Veh; # P < 0.05 versus G/MCM + HOE. (C) Histograms summarizing glioma cell viability after 48h of MCM stimulations. Data are mean ± SEM. n = 4, *P < 0.05 versus C/MCM + Veh; # P < 0.05 versus G/MCM + HOE.