Figure 2.
miR-200b regulates the expression/activation of cell cycle regulators and induces G2-phase arrest in ESCC cells. (A, B) Flow cytometry was performed to determine the effect of miR-200b on ESCC cells 36h after transfection. Control cells were transfected with a non-targeting negative control RNA. (C) ESCC cells transfected with miR-200b mimic/control for 36h were double stained with propidium iodide and phospho-Histone H3S10, then flow cytometry was performed. (D) Confocal microscopy was performed to determine the impact of miR-200b on mitosis. KYSE150 cells transfected with miR-200b mimic/control for 36h were used in this assay, representative images are shown. Scale bars: 5 µm. (E) Western blot assay was performed to determine the impact of miR-200b on the expression of various cell cycle regulators 36h after transfection. Actin served as a loading control. Representative results are shown, and data are presented as mean ± SD. *P < 0.05, ***P < 0.001, Student’s t test.