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. 2016 Aug 4;37(9):858–869. doi: 10.1093/carcin/bgw079

Figure 5.

Figure 5.

The biological function of the miR-200b/PAF axis in ESCC cells. (A–C) EC109 and KYSE150 were transfected with PAF siRNA or a negative control siRNA, then Western blot assay was conducted 48h after transfection (A), cell growth assay was measured using MTS assay 72h after transfection (B) and clonogenic assay was performed 48h after transfection (C). (D–F) EC109 and KYSE150 were co-transfected with the indicated plasmids and miR-200b mimics or a negative control miRNA (Cont.), then Western blot assay was conducted 48h after transfection (D), cell growth assay was measured using MTS assay 72h after transfection (E) and clonogenic assay was performed 48h after transfection (F). (G) Dual luciferase reporter assay was performed to measure the TopFlash reporter activity. (H, I) Real-time PCR assay was conducted to measure the mRNA expression of Axin2 and LEF1. GAPDH was used as a loading control for real-time PCR, and Actin was used as a loading control for Western blots. Representative results are shown, and data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test.