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. 2016 Mar 16;3:16005. doi: 10.1038/mto.2016.5

Figure 3.

Figure 3

Effects of N-myc on the establishment of the antiviral state. TET-21N and TET-21C cells were either pretreated or not with 1 μg/ml doxycycline (Dox +/-) and incubated for 48 hours. Cells were then treated with either 200 U/ml IFN-β (a,b) or extracellular poly I:C (c,d) for 6 and 12 hours, respectively, followed by virus infection at multiplicity of infection (MOI) of 5. Viability was determined with Alamar Blue at indicated time points. NB cell lines were transfected with 2.5 ng (in a 24-well plate format) or treated with 10 μg/ml poly I:C for 12 hours. Alternatively, cells were treated with exogenous IFN-β (200 U/ml) for 6 hours. Cells were subsequently infected with VSVΔM51 at MOI of 5 (e,f). Viability was determined with Alamar Blue. Error bars correspond to mean ± standard deviation of the mean from three replicates. Dox (-), no doxycycline added. Dox (+), doxycycline added.