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. 2016 Mar 16;3:16005. doi: 10.1038/mto.2016.5

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Differential expression of DDIT-3. (a) TET21 cells were either treated or not with doxycycline for 48 hours and infected with VSVΔM51 at multiplicity of infection (MOI) of 0.5 for 12, 18, and 24 hours. Alternatively, cells were treated with 1 μg/ml tunicamycin (an ER poison) as controls for DDIT-3/CHOP expression. Expression of DDIT-3/CHOP was analyzed by western blot (a). NB cell lines were either infected at MOI of 0.1 or treated with tunicamycin for 15 hours. RNA was extracted and the expression of DDIT-3/CHOP was analyzed by RT-PCR (b). Dox (-), no doxycycline added. Dox (+), doxycycline added.