Figure 4. Sphingoid bases decrease CLS.
A.-D. CLS assays of yeast cells. Gal1Δ cells overexpressing either Ypc1p or Ypc1pC27F A., WT vs. Lcb4ΔLcb5Δ cells B., WT cells treated with ethanol or PHS (10uM) C., and WT vs. Ydc1ΔYpc1Δ D. were subjected to CLS assays as described in Materials and Methods. Survival rates of yeast cultures were assayed in triplicate. The data represent the mean ± SD of fold changes in survival rates at indicated time points vs. those at the day 0 time point ( n = 5), *p < 0.05.