Skip to main content
. 2016 Jan 27;7(16):21315–21331. doi: 10.18632/oncotarget.7040

Figure 3. Metformin antagonizes 17β-estradiol-induced anti-apoptosis effect in endometrial adenocarcinoma cells.

Figure 3

Ishikawa cells A. and KLE cells B. were treated with metformin, E2, or both for 48 h. Cells were harvested and stained with PE Annexin V and 7-AAD, and cell apoptosis was analyzed using flow cytometry. Representative images were showed. The percentages of PE Annexin V + /7-AAD − cells (early apoptosis) were plotted. The data are presented as the mean ± SD of three replicates per group. E2: 17β-estradiol; Met: metformin. * p < 0.05, ** p < 0.01 vs. untreated cells.