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. 2016 Jan 27;7(16):21315–21331. doi: 10.18632/oncotarget.7040

Figure 9. βKlotho expression and metformin show synergetic inhibitory effects on the proliferation and EMT in Ishikawa cells.

Figure 9

The EV and βKlotho transfected Ishikawa cells were treated with or without 10 mM metformin, CCK-8 assays A. and colony formation assays B. were performed at indicated times. Scale bar: 1 cm. C. Ishikawa cells transfected with either EV or βKlotho were treated with or without 10 mM metformin for 48h. Western blot was performed to detect the expression of E-cadherin, N-cadherin, Slug, and Snail. β-actin was used as a loading control. The data are presented as the mean ± SD of three replicates per group. Met: metformin. *p < 0.05, ** p < 0.01.