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. 2016 Feb 19;7(16):21510–21526. doi: 10.18632/oncotarget.7517

Figure 4. MET proto-oncogene is a target of miR-329 at specific 3′-UTR site.

Figure 4

A. pmiR-RB-REPORT TM dual-luciferase reporter vector; The 3′-UTR of MET harbors one miR-329 cognate site; Relative luciferase activity of reporter plasmids carrying wild-type or mutant MET 3′-UTR in A549 and H1299 cells co-transfected with negative control (NC) or miR-329 mimic. B. Scatter plots showing the inverse association between miR-329 level and MET mRNA expression. C. Western-blot of c-Met protein in A549 and H1299 cells after transfection; statistical analysis of western-blot; qRT-PCR of c-Met mRNA in A549 and H1299 cells after transfection. D. c-Met immunofluorescent staining in A549 and H1299 cells after transfection. Bar = 10 μm. Assays were performed in triplicate. Means ± SEM are shown. Statistical analysis was conducted using student t-test.