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. 2016 Feb 19;7(16):21510–21526. doi: 10.18632/oncotarget.7517

Figure 5. Ectopic expression of miR-329 suppresses tumor growth in vivo and vitro.

Figure 5

A. Force-expression of miR-329 suppresses tumor growth in vivo. Tumor volume and weight in nude mice. Each group contained six mice (n = 6); the data are presented as the mean ± SEM; *p < 0.001, compared with the NC group; The expression of c-Met protein and mRNA in nude mice. Assays were performed in triplicate. Means ± SEM are shown. Statistical analysis was conducted using student t-test. B. Ectopic expression of miR-329 inhibits NSCLC cell growth. A549 and H1299 cells were transfected with miR-329 mimic and mimic NC, miR-329 inhibitor and inhibitor NC, for forty-eight hours, respectively. Shown are representative photomicrographs of BrdU staining in A549 and H1299 cells after transfection. Bar = 100 μm; Statistical analysis of BrdU staining; CCK8 assays of A549 and H1299 cells after transfected with miR-329 mimic, miR-329 mimic NC, miR-329 inhibitor, miR-329 inhibitor NC; Shown are representative photomicrographs of colony formation assay after transfected with miR-329 mimic, miR-329 mimic NC, miR-329 inhibitor or miR-329 inhibitor NC for ten days; Statistical analysis of colony formation assay. C. Western-blot of cyclin D1 protein in A549 and H1299 cells after transfection; Statistical analysis of western-blot of cyclin D1 protein; qRT-PCR of cyclin D1, cyclin D2, p57 and p21 mRNA in A549 and H1299 cells after transfection. Assays were performed in triplicate. Means ± SEM are shown. Statistical analysis was conducted using student t-test.