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. 2016 Feb 19;7(16):21510–21526. doi: 10.18632/oncotarget.7517

Figure 7. Ectopic expression of miR-329 promotes apoptosis in A549 and H1299 cells.

Figure 7

A. Quantitative representation of casepase-3 and casepase-7 activity in A549 and H1299 cells transfected with related miRNAs for forty eight hours. B. Shown are representative photomicrographs of cells dual-stained with annexin-FITC/PI. Bar = 10 μm. C. Shown are representative photomicrographs of flow cytometric analysis; Statistical analysis of flow cytometric analysis. D. Western-blot of Bcl2 protein in A549 and H1299 cells after transfection; Statistical analysis of western-blot; qRT-PCR of Bcl2 mRNA in A549 and H1299 cells after transfection; Western-blot of cleaved-caspase-3 protein in A549 and H1299 cells after transfection; Statistical analysis of western-blot. Assays were performed in triplicate Means ± SEM are shown. Statistical analysis was conducted using student t-test.