Figure 10. Effect of AQ1 on c-KIT mRNA and protein expression in α155 and HMC1.2 cell lines.

The c-KIT mRNA levels panels A-B. were measured using a qPCR assay, and data (arithmetic means ± S.D.) are expressed as n-fold change (arbitrary units, a. u.) normalized to the RQ of control cells at each time (T₆, T₁₂) to which an arbitrary value of 1 was assigned. Two-way ANOVA followed by Bonferroni post-test was used to assess statistical differences between doses and time of treatment. ^***: significant differences (P<0.001). The c-kit protein amounts panels C-D. were measured 48 hours post-exposure by flow cytometry and data are expressed as n-fold change (%) to the mFI of not treated cells. One-way ANOVA followed by Bonferroni post-test was used to assess statistical differences between cell treated with AQ1 and those treated with the vehicle (DMSO). ^*,**: P<0.05; P<0.01.