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. 2016 Mar 7;7(16):21713–21727. doi: 10.18632/oncotarget.7964

Figure 1. Notch expression in bone marrow MSCs isolated from healthy donors (hBM-MSCs) and AML patients (hBM-MSC*).

Figure 1

A. Flow cytometry analysis of MSCs for expression of hematopoetic, endothelial and mesenchymal markers. B. Oil Red O staining (upper panel) and Alizarin Red staining (lower panel) of mesenchymal cells grown during 21 days in adypocyte and osteogenic differentiation media, respectively. C. Relative quantification of protein expression after densitometry analysis of immunoblots. Protein expression levels were normalized to expression levels of GAPDH, data are expressed as the mean ± SEM of 12 patients and 12 healthy donors *p < 0.05, **p < 0.01. HEK-293 cell line was used as positive control. NTM: Notch Trans-Membrane domain; FL: Full Length; EC: Notch Extracellular Cleaved domain. As indicated in the datasheet, anti-Notch4 detected 3 different isoforms (a, b and c).