Figure 2. High accuracy of the plasma-based direct quantification of circulating miRNAs.

(A) Quantitative analysis of miR-16 from 1, 4 and 8 μl of plasma sample using methods M3. (B) Ct values in A analysis. (C) The miR-16 abundance showed a linear positive correlation with the plasma volume with R² = 0.999. (D) Quantitative analysis of miR-16 from three different plasma samples (S1, S2 and S3) with 1:10 and 1:100 cDNA dilutions. (E) Actual Ct values and fold changes of miR-16 abundance in the three samples at cDNA dilution 1:10 vs 1:100. (F) miR-16 abundance showed 10.27 times more in 1:10 than 1:100 diluted cDNAs, which is consistent with cDNA concentration.