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. 2016 Mar 8;7(16):21865–21874. doi: 10.18632/oncotarget.7990

Figure 3. High specificity of the plasma-based direct quantification of circulating miRNAs.

Figure 3

(A) QRT-PCR analysis of an exogenous small RNA unisp6 which was added into purified RNA with 1:10 and 1:100 dilutions to cDNA. (B) Quantitative analysis of unisp6 abundance in A. (C) QRT-PCR analysis of exogenous small RNA unisp6 which was added into plasma with 1:10 and 1:100 dilutions to cDNA. (D) Quantitative analysis of unisp6 abundance in C. (E) Quantitative analysis of exogenous small RNA unisp6 which was added into plasma with volume 0, 1 and 4 μl. (F) Quantitative analysis of miR-16 in all cDNAs of E. (G) Quantitative analysis of 5s rRNA in all cDNAs of E.