Figure 4. High efficiency of the plasma-based direct quantification of circulating miRNAs.

(A) Details of the procedure for the RNA-based and plasma-based miRNA analysis for comparison of the two methods. (B–D) Comparison of the RNA-based and plasma-based analysis for circulating let-7b and miR-92a in three independent samples S1 (B), S2 (C) and S3 (D). E-F: Comparison of the RNA-based and plasma-based analysis for the different expression of let-7b (E) and miR-92a (F) in plasma samples S4 and S5. (G) ΔΔCt value of let-7b and miR-92abetween samples S4 and S5. H-I: The linear correlation with R² equals 1.0 between the RNA-based and plasma-based methods for the different expression analysis of let-7b (H) and miR-92a (I) in samples S4 and S5.