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. 2016 Mar 8;7(16):22339–22354. doi: 10.18632/oncotarget.7970

Figure 5. ZFX is a direct downstream target of miR-101.

Figure 5

A. Putative miR-101-binding sequence within the 3′UTR of ZFX mRNA. Mutations in the complementary site for the seed region of miR-101 in the 3′UTR of the ZFX gene are indicated. B. Reporter plasmids containing either the wild type 3′UTR or a mutated 3′UTR of the ZFX gene were co-transfected into 293T cells with an miR-101-encoding plasmid, and luciferase activity was measured. The data are presented as the mean ± SD of three experiments. C. ZFX mRNA expression after miR-101 transfection was detected by qRT-PCR. D. ZFX expression in miR-NC- and miR-101-transfected GBC cells was analyzed by western blot analysis. β-actin was used as a loading control. GBC cells were transfected with an miR-NC/empty vector, an miR-101/empty vector, or an miR-101/ZFX plasmid. E. Cell growth rates were determined using a CCK-8 proliferation assay (**P<0.01). F. Transwell migration and invasion assays were performed, and the results are shown in the right panel (**P<0.01). G. Wound healing assays were used to detect cell motility after transfection. The wound closure percentages are shown in the right panel (**P<0.01).