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. 2016 Mar 10;7(16):22605–22622. doi: 10.18632/oncotarget.8027

Figure 2. Validating colonization by Symbioflor-2 in a RenCa tumor model demonstrates persistence of colonization and intrinsic tumor specificity following systemic inoculation.

Figure 2

Wild-type mice received a subcutaneous injection of 1.5×106 RenCa tumor cells. “Symbioflor-2” constitutes strains G1/2, G3/10, G4/9, G5, G6/7, and G8 pooled at equal ratios. A. Colonization profile displaying CFU counts in blood (B), liver (L), spleen (S), and RenCa tumor (T) upon systemic inoculation of strain G1/2. B. Comparison of RenCa tumor colonization between Symbioflor-2, EcN, and SL7207 over 7 days of infection. C. Specificity of RenCa tumor colonization 7 dpi between Symbioflor-2, EcN and SL7207. D. IHC staining of consecutive RenCa tumor cross sections 48 hpi with Symbioflor-2. E. coli were stained using a specific antibody, MPO activity was indicative of neutrophil activity and regions of hypoxia were stained for presence of pimonidazole metabolites. V and N denote viable and necrotic regions of the tumor, respectively. Representative images are displayed. Scale bar corresponds to 100 μm. N=3-5. Median ± range.