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. 2016 Mar 10;7(16):22605–22622. doi: 10.18632/oncotarget.8027

Figure 4. Symbioflor-2 fitness parameters; biofilm forming capacity, complement sensitivity, phagocytosis and intracellular survival.

Figure 4

A. In vitro biofilm forming capacity of representative Symbioflor-2 strains and EcN developed over 20 days on spiked agar plates. Bottom and top rows represent positively and negatively scanned biofilms, respectively. B. Quantification of static biofilm formation by Symbioflor-2 strains, EcN and SL7207 relative to a biofilm-positive Pseudomonas aeruginosa control strain PA14 (100%), as determined through crystal violet staining of a 48 h culture deposit. N=8. C. Ultrathin sections of a Symbioflor-2 colonized CT26 tumor section isolated 48 hpi. Arrow heads point to the colonizing bacteria. Representative images are displayed. D. Bacterial uptake by ex vivo matured BMDMs, as determined through relative comparison of intracellular bacteria 1 hpi in a co-culture gentamicin survival assay. E. Intracellular survival/ replication of the bacteria in BMDMs as determined through intracellular CFU counts 18 hpi and expressed relative to levels of uptake 1 hpi. N=5. Wild-type Salmonella strain ATCC14028 (WT S.T.) was included as a positive control. F. Bacterial susceptibility to lysis by human complement. Treatment with heat inactivated (HI) human complement constitutes a control for viable bacteria and relative inocula. G. Sensitivity to canine complement lysis for G1/2, G3/10 and EcN. N=5. Mean ± SEM and median ± range were deployed on linear and log scales, respectively.