Figure 8. ERG regulates p53 signaling.
LnTE3 cells (2.5 × 105) were either untreated or treated with doxycycline (1μg/ml) and transfected with pre miR-449a or siSIRT1 RNA and respective controls (20nM each) using siPORT transfection reagent on the following day. a. Total and acetylated p53 proteins levels were analyzed by immunoblot in dox-treated LnTE3 cells incubated with pre miR-449a (48 h). b. SIRT1, ERG, p53 (total and acetylated) protein expression was analyzed by immunoblot in dox-treated LnTE3 cells incubated with siSIRT (48 h). The relative quantification are indicated below the corresponding immunoblot. The data is representative of three independent experiments (mean ± SD).