Figure 2. CAS is essential for migration of HCC cells in vitro.

(A) HLE and HLF cells were treated either with a control siRNA (ctrl) or two CAS specific siRNAs (CAS#1 and CAS#2) and cell extracts were immunoblotted with the indicated antibodies. (B) HLE (left panel) and HLF (right panel) cells were treated as described in (A). 48 h after transfection proliferation was inhibited using mitomycin C and confluent cell monolayer was scratched. Migration was measured by monitoring the closure of the “scratch-wound” using life cell imaging. Time course diagrams show a representative experiment, respectively. (C) Corresponding pictures illustrate the migration assay performed in HLF cells.