Figure 4. Promoter-specific effect of the saRNAs on DPYSL3 expression.
A. Scheme of DPYSL3v2 promoter-driven luciferase reporters. Δv2-9 or Δv2-5 indicates a fragment deletion of the saRNA-targeted site. B-D PC-3 cells plated in 6-well plates were infected with lentiviral particle containing the parental reporter (B & C) or individual mutant reporter (D) and then transfected with the saRNAs at a dose of 10 nM B. or as indicated in the panel C and D.. The scramble saRNA was used at 10 nM. Luciferase activity was measured one day later and the fold induction was calculated against the scramble control. Data were shown as the average and the error bar represents the SEM. The asterisk indicates a significant difference compared to the control (p < 0.05, student's t-test).