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. 2016 Jul 8;311(2):H433–H444. doi: 10.1152/ajpheart.00176.2016

Fig. 4.

Fig. 4.

Effect of blockade of MC3/4Rs in the DMN and PVN on ARCN responses. A and B: unilateral microinjections of NMDA (10 mM, 30 nl) into the ARCN elicited increases in integrated IBATSNA and TBAT (open bars). Twenty minutes later, ipsilateral DMN was identified by microinjections of NMDA, and after NMDA effects subsided, SHU9119 (2 mM) was microinjected into the DMN (arrow); blockade of MC3/4Rs in the DMN by SHU9119 did not elicit significant changes in IBATSNA and TBAT (not shown). NMDA was again microinjected into the ARCN within 2 or 3 min; increases in IBATSNA and TBAT were significantly attenuated by MC3/4R blockade in the DMN (black bars). In the same animals, microinjections of aCSF (50 nl) into the DMN did not alter significantly NMDA-induced responses in IBATSNA and TBAT (not shown). C and D: procedures used were identical to those mentioned in A and B, except that SHU9119 and aCSF were microinjected into the PVN previously identified by microinjections of NMDA. Microinjections of SHU9119, but not microinjections of aCSF, into the PVN significantly attenuated increases in IBATSNA and TBAT elicited by ARCN stimulation. *P < 0.05; **P < 0.01; ***P < 0.001.