Mitochondrial respiration in adipose tissue is not increased in previously trained mice fed a high-fat diet. Mitochondrial respiration measured by high-resolution respirometry in epididymal white adipose tissue (A) and interscapular brown adipose tissue (B). Gray bars denote SED HFD, while white bars denote TR HFD. P, pyruvate; M, malate; D, ADP; G, glutamate; S, succinate; oligo, oligomycin; DNP, dinitrophenol; JO2, rate of oxygen consumption. Data are presented as means ± SE; n = 7. *Significantly different from SED, P < 0.05.