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. 2016 Sep 1;11(9):e0161479. doi: 10.1371/journal.pone.0161479

Fig 8. HACs interrupt type I collagen synthesis after 7 days of dynamic culture.

Fig 8

Collagen sponges seeded with HACs were cultured in either static or dynamic conditions (program 1, n = 2) in the presence of BIT. At day 7 (D7), day 14 (D14), and day 21 (D21), IHC analyses were performed to localize type II and type I collagen. In dynamic conditions, note the absence of type I collagen in cell cytoplasm at day 14 and 21, as attested by the lack of brown staining (red arrows). Type II collagen synthesis was instead maintained during the whole culture, as demonstrated by cell cytoplasm staining (yellow arrows) from day 7 to 21 (scale bars 10 μm).