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NE does not modify nitrite conversion to nitrate. Microglia cells were incubated with LPS (1 ng/ml) plus or minus 10 μM NE. After 24 hr, the levels of nitrite (open bars) and nitrate (filled bars) in the cell culture media were determined. ***, P < 0.001 versus LPS alone (NO2); §§, P < 0.05 versus LPS alone (NO3). The ratio of nitrite to nitrate in LPS treated cells was 0.91 ± 0.02, and in LPS/NE treated cells was 0.79 ± 0.03 (n = 3).
