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Microglial NOS2 mRNA is reduced by NE. Total cytosolic RNA was prepared from control microglia, or microglia incubated with LPS (open bars) or LPS plus 1 μM NE (filled bars), and used for Q-PCR analysis of NOS2 mRNA. Data are means ± s.e.m of 2 replicates per group, and are the values calculated for NOS2 mRNA levels relative to control (non-stimulated) cells. *** and **, P < 0.001 and 0.01 versus LPS alone; two-way ANOVA followed by Bonferroni's post hoc test.
