TABLE 1.
Enzymatic activities for peptidyl-MCA substrates
| Peptide substratea | Cleavage activity (nkat/ml)
|
|
|---|---|---|
| P4 P3 P2 P1↓ P1′ | pNGA142 | pNAKX1 |
| Boc-Gln-Arg-Arg-MCA | 0.07 | 3.47 |
| Boc-Leu-Lys-Arg-MCA | 0.06 | 3.15 |
| Boc-Leu-Arg-Arg-MCA | 0.07 | 2.91 |
| Boc-Arg-Val-Arg-Arg-MCA | 0.05 | 2.39 |
| Boc-Val-Pro-Arg-MCA | 0.04 | 1.74 |
| Boc-Gly-Lys-Arg-MCA | 0.03 | 1.30 |
| Boc-Gly-Arg-Arg-MCA | 0.01 | 0.56 |
| Boc-Leu-Ser-Thr-Arg-MCA | 0.02 | 0.55 |
| Boc-Leu-Gly-Arg-MCA | 0.00 | 0.11 |
| Boc-Gln-Gly-Arg-MCA | 0.01 | 0.20 |
| Boc-Glu-Lys-Lys-MCA | 0.00 | 0.01 |
a
The arrow indicates the cleavage site by A. oryzae KexB. Boc, t-butyloxycarbonyl-; MCA, 4-methylcoumaryl-7-amide. Basic amino acids are in bold.