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. 2016 Sep 1;11(9):e0161859. doi: 10.1371/journal.pone.0161859

Fig 2. CCND2 expression and promoter methylation in RCC cell lines before and after demethylation treament.

Fig 2

(A) Reverse transcription PCR (RT-PCR) was used to analysis CCND2 expression in RCC cell lines and HK-2 cell with or without demethylation drugs, AZA: 5-aza-2’-deoxycytidine; A+T: AZA + TSA (trichostatin A); -ve represents negative control. (B) Methylation specific PCR detected the corresponding methylation status in RCC cell lines. (C) Qualitative analysis of CCND2 mRNA expression in 786-O and 769P cells after demethylation treatment by Real-time PCR, * represents p<0.05.