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. 2016 Sep 1;12(9):e1006293. doi: 10.1371/journal.pgen.1006293

Fig 2. Dmrt1 mutant undifferentiated spermatogonia can differentiate.

Fig 2

IF of sectioned control (A-C, G-I, M-O) and conditional mutant (D-F, J-L, P-R) seminiferous tubules 10 and 15 days post-tamoxifen (DPT) detecting tdTomato (red) and three stage-specific germ cell markers (green). DAPI (blue) stains DNA. Anti-PLZF (A-F) was used to detect undifferentiated spermatogonia, anti-SOHLH1 (G-L) for differentiating spermatogonia and anti-SYCP3 (M-R) for meiotic cells. Double-positive cells are labeled by white arrowheads. Scale bars: 20μm.