Fig 3. Dmrt1 is required in undifferentiated spermatogonia to maintain spermatogenesis.
(A-H) IF of sectioned testes showing tdTomato (red) at 10, 15, 21 and 40 DPT. DAPI DNA stain (blue). In controls (A-D) tdTomato-positive cells are maintained for a full round of spermatogenesis (40d). In mutant testes (E-H) tdTomato-positive cells were progressively lost, with more than 60% of tubules (N = 101/167) having no tdTomato-positive cells by 40 DPT (indicated by asterisks). Scale bars: 20 μm.