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. 2016 Sep 1;12(9):e1006293. doi: 10.1371/journal.pgen.1006293

Fig 4. Dmrt1 mutant SSCs are not maintained.

Fig 4

(A-F) IF of sectioned 15 DPT testes showing tdTomato (red) and GFP (green). DAPI DNA stain (blue). All tdTomato/GFP double-positive cells are labeled with white arrowheads. In control testes (A-C) many tdTomato/GFP double-positive cells were present, while in mutant testes (D-F) double-positive cells were rare. Higher magnifications are shown in insets. GFP-positive meiotic cells represent previously described ectopic expression of the Id4-Gfp transgene (11). (G, H) IF of wholemount seminiferous tubules 15 DPT showing tdTomato (red) and GFP (green). In control (G) all tdTomato-positive cells were GFP-positive (white arrowhead; inset), while in mutant (H), GFP-positive cells almost all lacked tdTomato (yellow arrowhead; inset) indicating that they were wild-type. Scale bars: 20μm. (I) Quantification of Id4-GFP and tdTomato double-positive cells in control and mutant testes 10 (yellow) and 15 (blue) DPT. Values are average from >300 tubules. Error bars indicate standard deviation. * indicates P < 0.05 (Student’s T test). ** indicates P<0.005.