Fig 8. Dmrt1 is required for SSC regeneration.
IF of sectioned adult testes showing tdTomato (red) and GFP (green) 60 days after busulfan treatment (20mg/kg). DAPI DNA stain (blue). In Dmrt1flox/+; Ngn3-cre/+; tdTomato; Id4-Gfp control testes (A) tdTomato and GFP double-positive cells were observed after 60 days (inset) but in Dmrt1flox/flox; Ngn3-cre/+; tdTomato; Id4-Gfp mutant (B) no double-positive cells were observed. Scale bars: 20μm. (C) Quantification of Id4-GFP and Id4-GFP/tdTomato double-positive cells in control and mutant testes. Double-positive cells are shown in yellow, GFP-positive cells are shown in green. Values are average from >200 tubule sections. Error bars indicate standard deviation. *** indicates P < 0.0005 (Student’s T test). (D) Model of DMRT1 during SSC homeostasis. SSCs are indicated in green, NGN3-positive undifferentiated spermatogonia are indicated in red.