TABLE 3.
Oligonucleotides used in this study
| Primer | Sequencea |
|---|---|
| GPR1-1+ | TCCTACAAGAATATTGACTCAT |
| GPR1-1− | GGGTAATTGGAGCAATGG |
| GPR1-2+ | AA CCGGACCTAATATCAATAGC |
| GPR1-2− | CTGGCAACAATCAATGGGACAAA |
| GPR1-3+ | TG GGAAAAATGCTGCTGGTAATGG |
| GPR1-3− | G AATAATTCTTTGTCGTCTTCGG |
| GPR1-4+ | CTATG GTGCAGGCATCAATTG |
| GPR1-4− | T CATTGGGGGTCCTTTTTTGAG |
| GPA2+ | GTATCTGTTTCACTTGCAACAGTC |
| GPA2− | ACAACAGCTTGAATACGTATCGAC |
| Q355L+ | ATTTATTTGATGTTGGTGGTttAAGGTCAGAAAGAAAAAAATGG |
| Q355L− | CCATTTTTTTCTTTCTGACCTTaaACCACCAACATCAAATAAAT |
| ADH1-NcoI+ | CAAATCGTCTACCAGAAGTTATGC |
| ADH1-NcoI− |
 CGAGAACTCGGAAAAAAAAAGTGC |
| ADH1-NdeI+ | ACACTTCTAAACAAACCAATCAAT |
| ADH1-NdeI− | AA AATTGTTTTTGTATTTGTTGTTGTTGTTG |
| TRP1+ | CGTTGAGGTTTCCGTTGTTTTC |
| TRP1− | CTGCCACCATAGTCGTAAGAGG |
| HWP1+ | GTGAAACAGAGGAAGCTCTTATTC |
| HWP1− | TTCAGAAGTAGTAGTTGTAGCAGG |
| ECE1+ | TATGGGTATTCTTGGCAACATTCC |
| ECE1− | TCAATACCGACAGTTTCAATGCTC |
| ACT1+ | AACATCCAGTTTTGTTGACCGAAG |
| ACT1− | ACTTCATGATGGAGTTGAAAGTGG |
a
The introduced restriction site is underlined; changed nucleotides are in lowercase.