Table 2. List of sequences of primers and PNA probes used in real-time PCR.

Gene	Mutation	Direct-ion	Primer		PNA Probe
			Primer Sequence (5' - 3')	Size (bp)	Probe Sequence (5' - 3')	Fluo-resce-nce
GJB2	p.V37I	F	GGGGTGTGAACAAACACTCCA	161	CCACAACGAGGATC	FAM
		R	ATCTCCCCACACCTCCTTTGC
	c.235delC	F	ACGATCACTACTTCCCCATCTCC	145	CAGGGCCCATAGCCG	Cy5
	c.299delAT	R	CTCTTTATCTCCCCCTTGATGAAC		CATGTCTCCGGTA	HEX
	p.R143W	F	GGTGGACCTACACAAGCAGCA	90	GATGACCCGGAAGAA	Texas Red
		R	TGGAGAAGCCGTCGTACATGA
SLC16A4	p.T410M	F	TTTGGGATCAGCAACATCTTCTC	154	TCCCGCACGGCCGTC	FAM
		R	CCATTCCTCGACTTGTTCTCTGA		CCGCATGG	FAM
	p.L676Q	F	CAATCCATAGCCTTGTGCTTGAC	151	AGATCACTGCGGG	HEX
		R	TTGCAATACTGGACAACCCACAT
	p.H723R	F	AGCCTGGGCAATAGAATGAGACT	229	GGTCCATGATGCTA	Texas Red
		R	AAATGGAACCTTGACCCTCTTGA
	c.IVS7-2A>G	F	CACAAAATCCCAGTCCCTATTCC	221	TTTTATTTCAGACGATAA	Cy5
		R	CCCTTGGGATGGATTTAACAATG
MTRNR1	1555A>G	F	GGTCGAAGGTGGATTTAGCAG	202	ACGACTTGTCTCCTCTA	HEX
		R	GCTACACTCTGGTTCGTCCAA
TMPRSS3	p.A306T	F	ATTTCAGCTTGTACCTCCCCAAG	189	ATGACATCGCCCTTATG	Cy5
		R	ACCCAGATGTACCATTGAACGTG
CDH23	p.P240L	F	ACTTGGCCATCATCATCACAGAT	162	AACCTGCCTTACAGC	Texas Red
		R	CCCTAACAGGAGCTCAGAAGGAA

F: forward direction, R: reverse direction