Table 3. List of primer sequences used in Sanger sequencing.

Gene	Mutation	Direction	Primer
			Primer Sequence (5' - 3')	Size (bp)
GJB2	p.V37I, c.235delC, c.299delAT, p.R143W	F	GGGGTGTGAACAAACACTCCA	456
		R	TGGAGAAGCCGTCGTACATGA
SLC16A4	p.T410M	F	GGATCAGCAACATCTTCTCAGGA	158
		R	CTCTGTTGCCATTCCTCGACTT
	p.L676Q	F	CAATCCATAGCCTTGTGCTTGAC	151
		R	TTGCAATACTGGACAACCCACAT
	p.H723R	F	AGCCTGGGCAATAGAATGAGACT	229
		R	AAATGGAACCTTGACCCTCTTGA
	c.IVS7-2A>G	F	CACAAAATCCCAGTCCCTATTCC	221
		R	CCCTTGGGATGGATTTAACAATG
MTRNR1	1555A>G	F	GGTCGAAGGTGGATTTAGCAG	202
		R	GCTACACTCTGGTTCGTCCAA
TMPRSS3	p.A306T	F	ATTTCAGCTTGTACCTCCCCAAG	189
		R	ACCCAGATGTACCATTGAACGTG
CDH23	p.P240L	F	ACTTGGCCATCATCATCACAGAT	162
		R	CCCTAACAGGAGCTCAGAAGGAA

F: forward direction, R: reverse direction