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| Sanger sequencing | Total | |||
|---|---|---|---|---|
| Positive | Negative | |||
| Real-time PCR kit(U-TOP™ HL Genotyping Kit) | Positive | 127 (a) | 0 (b) | 127 |
| Negative | 0 (c) | 144 (d) | 144 | |
| Total | 127 | 144 | 271 | |
The detection of 1 or more mutations was scored as a positive result, and wild type sequences were scored as negative.
