Fig 3. Validation of RNA-Seq results was performed by qRT-PCR analysis on 5 randomly chosen genes from the list of differentially expressed genes.

CEACAM5 also was analysed as positive control. Results obtained by RNA-Seq and qRT-PCR methods show high accordance. Each qRT-PCR was performed with three replicates. All data are means ± SD. (*p < 0.05; **p < 0.01, ***p < 0.001, one-way ANOVA for qRT-PCR and FDR-adjusted exact test for RNA-Seq).