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. 2016 Sep 1;11(9):e0162357. doi: 10.1371/journal.pone.0162357

Fig 3. Expression of type I collagen, SMA, and CCN2 in scleroderma lung fibroblasts (LF) transfected with MET WT and MET D1398G.

Fig 3

Non-transfected (NT) and transfected scleroderma (SSc) lung fibroblasts (LF) were serum-starved for 24 hours followed by incubation for 48 hours with recombinant HGF (50 ng/ml). The cells were collected with lysis buffer and analyzed by Western blot with indicated antibodies including MET as a transfection efficiency control. Anti-β-actin antibody was used as a loading control. B. Quantitative results of densitometric analysis of immunoblots. Values are the mean and SD from four independent experiments.